Was out looking for frogs, toads or other interesting nocturnal critters to use my 100 2.8 Macro on, when I came across this guy. I coaxed him into a, uh, temporary "glass house" for a quick photoshoot indoors. Don't worry, he was released back into the night shortly after. Taken with a tripod, ISO 1600. Flash was a no-go due to the glass.
In the indoor light (but not bright enough to allow higher f-ratio for greater depth-of-field). 1/20 sec, f/2.8:
And with the lights mostly off (some ambient), brightened considerably. 2 sec exposure @ f/4. Sorry the shot is so blurry - oddly enough the bugs won't glow when the lights are on:
The part that glows is quite visible in the normal-light shot, and looks almost like the glow-in-the-dark fluorescent paint found on old watches.