This is the vertical set, although I did shoot them with the camera horizontal but framed so that I could turn them in post. Sometimes it's easier for me to shoot a portrait with the camera in landscape. As with the last set the apparent depth is due to way that I angle the camera and not a function of the lens. According to the MP-E 65mm manual the depth of field for the 5x shot was .19mm.
Technique: I found this European Wool carder bee snoozing on a Lavender stalk before sunset and woke up early the next morning to photograph it. I placed an artificial flower in the background to keep it from being black.
Tech Specs: Canon 80D (F11, 1/250, ISO 200 with highlight tone priority) + a Canon MP-E 65mm macro lens (over 2x to 5x) + a diffused MT-24EX (both flash heads on the Canon flash mount, E-TTL metering with -1 2/3 FEC). These are single, uncropped, frames taken hand held.