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Thread started 02 Aug 2011 (Tuesday) 09:52
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Hibiscus Pollen

 
canonloader
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Aug 02, 2011 09:52 |  #1

This ought to make you sneeze.

1. This is one of 8 or 10 pads on the Pistil of the flower. This is what collects the pollen from other flowers, then fertilizes the plant. To the eye, it is smooth and looks like soft leather. About 40x.

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2. This is one of the stamens at about 40x. The stamen grows the pollen inside it and when the pollen is ripe, it bursts open. The pollen then waits for a hummingbird or bug to come to the flower, where it sticks to the bug or bird and is carried to other plants.
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3. Another Stamen. 40x.
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4. And another. The small stuff below the stamen here is some kind of liquid from the stamens, probably a glue to hold the pollen in place. It has smeared onto the coverslip and dried there.
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5. Some pollen grains. 40x.
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6. Another view of a Stamen. 40x.
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7. And a different angle. 40x.
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8. A closeup at about 100x.
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orionmystery
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Aug 02, 2011 10:50 |  #2

Cool shots Mitch, esp like 2 and 4.


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LindaB
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Aug 02, 2011 10:57 as a reply to  @ orionmystery's post |  #3

Fascinating shots Mitch.

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canonloader
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Aug 02, 2011 11:03 |  #4

Thanks Kurt and Linda. These shots were lit from the side, with a high intensity halogen fiber optic gooseneck illuminator. I used a small white opaque plastic cup that I cut the bottom out of to stick the lens through, then trimmed the height, so it would allow me to adjust the height of the stage on the microscope to achieve focus. Think diffuser.

Once the cup is in place, you aim the two fiber optic lights at the side of the cup. This allows the lighting you see here. If I had just used the light from the bottom like normal microscope lighting, the pollen is too thick for the light to come though and all you see is a silhouette. :)


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BasAndrews
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Aug 02, 2011 12:25 |  #5

Excellent, and interesting shots.

And I thought standard macro shots were complicated enough!.


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canonloader
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Aug 02, 2011 12:35 |  #6

Thanks Bas. It's not very difficult to view this stuff in the microscope, but getting the images was very hard to learn. If you've ever looked in a scope, the light that comes through is like the light from a pinhole. Not very much. That means lower shutter speeds, really low shutter speeds. These were all at 1/15" and ISO 400. Only possible, because pollen doesn't move. LOL

When shooting the little bugs, like rotifers and parameciums, you have to try some other tricks. :)


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Aug 02, 2011 13:38 |  #7

great colorful shots...liked all esp 1,2,4...


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Aug 02, 2011 21:57 |  #8

Thanks Rakesh. Yes, this one has a lot of nice colors to it. :)


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Aug 03, 2011 00:57 |  #9

Interesting stuff - think Hibiscus pollen is about the largest I've seen
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Aug 03, 2011 05:33 |  #10

Thanks Brian. I think you may be right. I know I did try to focus on it using the 20x objective and there just was not enough DOF to make it worth while on a round object like pollen. I have used the 20x on other pollen though. What struck me as different with these are the points on them. :)


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Aug 03, 2011 06:20 |  #11

No wonder pollen makes you sneeze. Looks like little tiny medieval morning stars!
Great series, as always, thanks for sharing!


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Aug 03, 2011 06:34 |  #12

Thanks for looking. This is the first spiked pollen I have seen. Somehow I got the impression most pollen looked like this, but not so. So far, most pollen I have seen looks like a raw wheat or barley grains in miniature. :)


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Aug 03, 2011 08:41 |  #13
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That is pretty fascinating stuff, Mitch, and goes to show that even the tiniest parts of flowers are beautiful :)




  
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canonloader
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Aug 03, 2011 09:07 |  #14

Thanks John. And this isn't even near high magnification. All these were taken at the lowest end of the spectrum, and even so, you can still see individual cells in that red column of the stamen, called the filament. I can see why the bugs eat the pollen though, it does kind of look like candy. :)


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Aug 03, 2011 11:29 |  #15

fab shots, makes me feel like sneezing just looking at it lol


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Hibiscus Pollen
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