These are Tiger Lily flower stalks, sliced very thin, then stained with Toluidine Blue O. This stain has the advantage of being relatively safe, very cheap and available and it stains different parts of the plant different colors.
Carboxylated polysaccharides such as pectic acids stain pinkish purple. Macromolecules with free phosphate groups such as nucleic acids stain purple or greenish blue. Polyphenolic compounds such as lignin and tannis stain green, greenish blue, or bright blue. Hydroxylated polysaccharides such as cellulose and starch are unstained.
This is my first try with this stain, but I am pretty happy with the results. I also tried a new technique with a double edge razor blade to slice some thin sections.
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