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Thread started 25 Aug 2011 (Thursday) 15:15
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Tiger Lily Stems Stained

 
canonloader
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Aug 25, 2011 15:15 |  #1

These are Tiger Lily flower stalks, sliced very thin, then stained with Toluidine Blue O. This stain has the advantage of being relatively safe, very cheap and available and it stains different parts of the plant different colors.

Carboxylated polysaccharides such as pectic acids stain pinkish purple. Macromolecules with free phosphate groups such as nucleic acids stain purple or greenish blue. Polyphenolic compounds such as lignin and tannis stain green, greenish blue, or bright blue. Hydroxylated polysaccharides such as cellulose and starch are unstained.

This is my first try with this stain, but I am pretty happy with the results. I also tried a new technique with a double edge razor blade to slice some thin sections.

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BasAndrews
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Aug 25, 2011 15:55 |  #2

Mitch.

very interesting and captivating images.

Bas.


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canonloader
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Aug 25, 2011 16:21 |  #3

Thanks for looking Bas. Been looking forward to trying stains. Most of them are very expensive, dangerous to use and hard to get them right when you do use them. Recently I found a few that are supposed to do a good job for a decent price and I think they are right. :)


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Shar824
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Aug 26, 2011 10:00 |  #4

I like these a lot Mitch, I can also see these printed large and hanging on a wall.


Sharon

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canonloader
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Aug 27, 2011 08:22 |  #5

Thanks for looking Sharon. It's a new option for the plant slices.


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jadrock
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Aug 29, 2011 04:41 |  #6

nice series...colorful patterns...


Regards,
Rakesh Jadhav
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canonloader
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Aug 29, 2011 08:05 |  #7

Thanks for looking Rakesh. This stain should make for some interesting images in the future.


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orionmystery
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Aug 29, 2011 10:14 |  #8

Wicked colors...like #1 best because of the nice shapes and patterns.


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canonloader
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Aug 29, 2011 10:37 |  #9

Thanks Kurt. I guess the stains are more for technical study of the plants and how they are put together, but since I found this stain, I'm doing it for the art side of it. :)


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Aug 29, 2011 16:08 |  #10

Those shots are kind of cool. Love the blue. Very calming.


I'm in Canada. Isn't that weird!

  
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canonloader
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Aug 29, 2011 16:14 |  #11

Thanks for looking AbPho. A first test with some new stains I found. I didn't really know what to expect, but I like what I got. :)


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Tiger Lily Stems Stained
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