I seem to have an outbreak of Mayorella bigemma in my aquarium, if that's what they really are. I didn't go to any particular trouble to collect these, just lifted the lid and skimmed my watchglass over the surface. In the first pipette, I got a good selection of these amoeba that kept me busy for awhile.
I used a Nikon 40x Plan Achromat PH3 DL lens over a Nikon Fluophot Phase Condenser on the Nikon Fluophot scope. [It's nice to have all the parts, finally].
Processing was done in CS5. It's cropped a bit and I cleaned up the background. The colors are what I get from the Phase Condenser. Not sure where the nice blue comes from, cause I can not see it after I set final focus. Once it is in focus, I flip a mirror and trip the shutter and flash with the mouse. No filters in the light path, other than the IR stuff built into the Fluophot. Sharpening is done using USM, 0.45, 0.3, and 0.0 to one or two of the color channels, usually the red and sometimes the green, and that's about it.
I did get video. It's rendering now. I will add the link when it's uploaded later today. Here is the video link
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1. Amoeba engulfing a small ciliate. The amoeba is only about 25µm.
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2. The ciliate has been engulfed. The video shows more of the process of digestion. It only took about 8 minutes for the ciliate to be digested.
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3. A second Amoeba. Is the black spot in these three images the nucleus?
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4. The second Amoeba as the slide dries up. They form a smooth round ball at the end.
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5. A third Amoeba.
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