I decided to try my hand at my first 5:1 stack with the MP-E 65. I purchased a neat little cactus at the local garden center on Sunday, and I took a shot of it from far enough out to give some scale and then from as close as possible to a single emerging flower.
First shot is natural light just after sunset. I don't remember the exact stats (did that one on Sunday), but it's a stack of something like 5 images shot from a tripod with hand focusing using the focus ring.
Second shot lighting is from two large studio soft boxes. Velbon macro rail, remote release, f/5.6, ISO 200, 32-shot stack in Zerene Stacker. That white line in the top right of the frame is a needle that I couldn't quite eliminate from the field of view. Even getting it down to just that one needle was difficult, because there are many small ones you can't really see in the first shot.
Thanks for looking 
At roughly 1:3
Single flower at 5:1.
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