ISO only affects the digital gain applied to the sensor (not sure at which point of the chain). When the light is low, signal to noise is lower, and when you amplify all of that you get to see the noise more than if your S/N was better. Noise doesn't make the image less sharp, but it makes it harder to see the sharpness behind all the crap, if you see what I mean.
Now, I can only speak about the 5D2 since that's the camera I researched before getting one, but I assume there's going to be similar work done on other cameras.
A number of people have graphed noise and DR against ISO, and found a few interesting things:
- noise levels at ISO 200 are exactly the same as at ISO 100.
- intermediate ISOs (in third stops: 125, 160, 250, 320, etc.) are derived from native ISOs (the 100, 200, etc. values) by taking the nearest native ISO and either pulling it up or pushing it down a third of a stop. As a result (*), the third-stops immediately above a full ISO are more noisy, and the third-stops immediately below are less so.
- since ISO 200 is the same as ISO 100, and since ISO 160 is cleaner than ISO 200, it stands to reason that ISO 160 is actually cleaner than ISO 100.
- in fact, even ISO 640 is as clean as ISO 100, which still kind of blows my mind.
- however, DR also suffers from higher ISO values. It turns out that you get the most DR out of ISO 100, with ISOs 320, 200, and 160 close behind in descending order.
- after ISO 1600, noise and DR have a linear relationship with ISO.
In conclusion:
- below ISO 800, you have no reason not to shoot at 160, 320, 640 instead of 100, 200, 400. In fact, unless you're going for the absolute least noise possible, you have no reason to shoot under 320.
- for some reason, ISO 1280, while way better than 1600, is worse than 800.
- at 1600 and higher, it's all linear.
HOWEVER, that applies to noise in absolute terms - not signal to noise. And that's where it gets fun: for the same scene, at ISO 100, you have twice as many photons as at ISO 200, since you're exposing twice as long. So while the noise at ISO 160 may be, what, 15% lower than at ISO 100, the signal to noise ratio shows a clear advantage for ISO 100 since the signal is, in fact, twice as strong.
So yeah, that's for the 5D2. It's all very interesting, but after all this I just choose to stick with the native ISOs. Maybe in certain fairly specific conditions you could squeeze a little extra juice from intermediate ISOs, but to be honest even at native 1600 on my 5D2 I don't feel noise is a real issue in practice, so why bother?
Sources:
http://home.comcast.net/~NikonD70/Charts/RN_ADU.htm
http://home.comcast.net/~NikonD70/Charts/PDR.htm
http://dokumentaryfotografr.blogspot.fr …o-settings-and-noise.html
http://www.pages.drexel.edu …kIITest/5DMarkIITest.html
http://photocascadia.wordpress.com …d-mark-ii-iso-noise-test/
http://shootintheshot.joshsilfen.com …canon-hd-dslr-native-iso/
(*) I say "as a result", but I don't actually understand why. I assume native ISO is gain applied at one part of the processing chain, and the third-stop gain adjustment is applied at some other point in a different fashion, because otherwise it'd just be a linear relation.