I had two of these, and actually just sold one of them today for $300. They were different from each other, both in IQ (very slight difference), and in focus. One of them, the one I kept, was dead on with my 7D with no micro adjustment. The other, I had to adjust to +11. I sold it to a guy with a 50D, and mentioned that it would probably have to be adjusted.
So yes, if you are going to buy one, bring your body and a laptop to the rendezvous. Open it up to 1.4 and spot focus on something with a lot of detail. Look at the image at 100% to see where it focused. If it's sharp in the right place, you're probably good.
All that said, I love the lens. I had two because I was playing with stereo images. I'm definitely keeping the one I have left.