I have recently been able to try both the EF70-300L and the EF 100-400 MkII for a week and I want to take time to post my impressions of both lenses. My experience may be useful to others.
First, to put these remarks in context, a few introductory comments. My main camera is a 7DMkII and my usual long lens is a Tamron 70-300 VC. I did not try either lens on a FF camera. A few weeks ago, I started a thread about these two lenses asking which one readers would choose and why. I left it pretty open ended in order to get a view of how people thought when arriving at their conclusions. For anyone interested, here's a link to that thread: https://photography-on-the.net …/showthread.php?t=1448702.
Before getting into specifics, let me say that both lens are a joy to use and the images they produce are beautiful. I would love to own either and may soon, with great difficulty, choose one. While I'm sure some differences can be found on an optical bench, in real world use, both seem exceptional to me. OK - on to specific observations.
- While the list price for the 100-400 is about $1000 more than that of the 70-300, a recent visit to the Canon Refurb Store showed a price of just under $1000 for the 70-300 and about $1400 for the 100-400.
- With the release of the 100-400 MkII, the price for used 70-300s is a bit soft and it may be possible to get quite a bargain used. Canon's on price may also be coming down a bit. The refurb price on the 70-300 is very good.
- The 70-300 fits into my normal bags so I can carry it with me and have it available when an opportunity presents itself. The 100-400 - especially with the tripod mount attached - is too large and will require either advanced planning to use or larger bags. Larger bags kind of defeats the purpose of traveling lighter.
- While both lenses focus fast and accurately, the 100-400 is faster.
- My 7DII image files are good enough and large enough to allow significant cropping when needed so the 100mm difference at the long end is, in my view, of less importance than other considerations. Besides 300mm is pretty long on a crop. On the other hand, the difference between 70mm and 100mm on the short end seems to me to be great enough to make the 70-300 more of a general purpose lens than the 100-400. Bear in mind that I did not use either lens on a FF camera where these differences might be more important.
- I personally have more difficulty picking up small moving subjects - such as BIF - at 400m than at 300mm. Practice and thoughtful use might change that with experience, but the 100-400 is a bit harder track moving subjects with.
- The 100-400 focuses closer so might have more useful macro functions.
- The zoom lock on the 100-400 stiffens zoom functions to prevent creep. The lock on the 70-300 actually locks the lens at 70mm and prevents any zoom function. However the 70-300 does not creep - the 100-400 (unlocked) does.
- The IS on the 70-300 is very effective but not so much as the IS on the 100-400.
- The 70 - 300 will not work with most Canon TC unless you set it a 300mm and tape it down so it won't move. A bit of a pain but workable.
- The focus ring on the 100-400 is smooth but not extremely light. And it is NOT where the user (at least this user) would hold the lens. The zoom ring, on the other hand, falls right to my grip easily.
- The focus ring on the 70-300 is VERY light and smooth. It is also, unfortunately, right under my hand when I hold the lens which leads to the possibility of inadvertently moving it slightly while manipulating the lens. The zoom ring is too far forward on the lens meaning I have to consciously reach for it. All this means that I must re-think my grip on the lens to avoid touching the focus ring and bring the zoom ring more easily to hand. Or buy a tripod mount for the lens (it does not come with one) which makes the lens much less likely to fit in a bag with other gear.
My general conclusion: I slightly prefer the 100-400 on the camera. I slightly prefer the 70-300 as part of a kit. Maybe the best choice is a 70-300L paired with the Sigma 150-600mm F5-6.3 DG OS HSM | Sport for when the long lens is REALLY needed. Or maybe the Canon EF 500mm f/4L IS II USM. Of course those are MUCH more expensive alternatives.
Hope someone finds this very long post useful. I would gladly own either lens. They are both a joy to use and produce excellent images. Please feel free to add to my comments.

. I use crop and FF and find the 70mm starting point much more useful than 100mm especially on FF
