Stacking removes random noise while keeping constant signal. The more you stack, the cleaner it will become as random noise is removed and the constant signal you keep stacking over and over will strength the signal to noise ratio, allowing you to pull more information from the signal you're capturing. Stacking increases your signal to noise ratio by the square root of the number of images stacked. That said, you get more signal from a longer exposure than a shorter exposure, and at the highest ISO possible you start losing out on the quality of that signal with really short exposure times. Basically, you're a lot better off at 30 seconds and ISO 1600 than you are at 2 seconds and ISO 25,600 (exposure is equivalent, but the longer exposure will yield greater signal quality). So this is my point about stressing that you should use the longest exposure time you can tolerate for your focal length and situation, then increase ISO until you get the histogram you need. It's possible to hit the maximum ISO to get there, but with wider focal-lengths it shouldn't be.
You don't have to use the highest ISO possible. You want to use the best combination of exposure time and ISO for your camera and your focal-length relative to the exposure you're trying to make (I assume this is static from a tripod, not on a tracker, so likely wide field like milky way). There's also a different histogram you're looking to achieve. You select the ISO needed to get you to that histogram with the exposure time you're limited to. There are more in depth ways to approach ISO and noise, such as read noise, etc, but if you're not on a tracker and you're limited to 30 seconds or less exposures from a static tripod, don't worry about that, and just focus on getting a good exposure and lots and lots of them to stack. It also matters what kind of light pollution you're working with in terms of how you want to expose your histogram (dark sky is different, from a well polluted street lamp infested sky as you hit a limit real fast under polluted skies).
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Exposure & histogram information (this is your bread & butter to get started and directly addresses what ISO you should be using based on the histogram):
https://jonrista.com …nal-noise-and-histograms/
All the information you may ever need to know about this subject (this goes into detail about why stacking is used and its benefits):
http://www.clarkvision.com …s/image-stacking-methods/
One of the best tutorials for processing DSO in general (milky way to DSO) from color dSLR files (this just teaches you how to process the night sky and draw detail from high signal to noise ratio final stacked images):
http://www.astronomersdoitinthedark.com/dslr_llrgb_tutorial.php
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So here's an example of my own that I learned from the above links and put into practice.
Using a Canon T4i (650D) at ISO 800 and my exposures are 240 seconds long (I'm on a tracker) with a 480mm F6 telescope (I'm shooting at F6 which is why I need that long exposure time, plus, longer exposure time means better signal quality). At my exposure time, I only needed ISO 800 to get the histogram I wanted, under a fairly dark sky where I am (minimal pollution) with a histogram that was around 1/3rd to 1/4th from the left or so. I did 21 exposures like this (21 x 240 seconds = 84 minutes of total exposure time). I only got 21 frames to work with, at longer exposure time, so my signal to noise ratio increase for doing 21 exposures is about 4.6 times more signal than I would have had in a single exposure un-stacked. I would have benefited from doing more, but an hour and a half basically of time just for the exposure was where I was comfortable at with the weather.
Here's the resulting stacked image results at 21 x 240 seconds through F6 and ISO 800 with the Canon T4i. Note the hisotgram (closer to 1/4th from the left, would have been better probably closer to 1/3rd from the left). You can see stars, but not much else. That's how its supposed to be. The real detail is buried in the signal that I'm stacking to get more of so I can stretch the histogram to reveal it. Because I stacked several images, the ISO noise is pretty much gone or at least not noticeable.
Image hosted by forum (
887241)
© MalVeauX [SHARE LINK] THIS IS A LOW QUALITY PREVIEW. Please log in to see the good quality stuff. Here's the same image with the histogram stretched so that I can see all that signal that was captured and stacked to see what feint details were hiding. See how the histogram is stretched now to fill most of it? Now you see all that feint nebulosity of the Rosette nebula coming through. This only works because I had quality signal (long exposure) and several exposures stacked increasing my S:R ratio by 4.6 times the amount a single image would have yielded. Also because random noise is stacked out, it helps keep some detail when stretching, instead of just stretching and amplifying noise.
Image hosted by forum (
887242)
© MalVeauX [SHARE LINK] THIS IS A LOW QUALITY PREVIEW. Please log in to see the good quality stuff. Here's that above stretched image then processed with color saturation. It's messy, but it shows you what was hidden in the signal that we did all this to get to in the first place:
IMAGE LINK: https://flic.kr/p/RnZhv5
RosetteNebula_NGC2238_01272017
by
Martin Wise
, on Flickr
And finally, here's my final processed image from the above, using the exact techniques in the video tutorial that I linked above on how to do color dSLR image processing:
IMAGE LINK: https://flic.kr/p/RgsUH1
NGC2238_ReProc_02042017
by
Martin Wise
, on Flickr
Very best,