Me and wife shot this yesterday... using off-camera Nikon flash connected via PC cord, (1/32nd power, about 3-4 feet away, positioned slightly to the front right, side lit ), ISO 800, Sigma 150mm macro lens, manual mode. The camera is positioned 45-degree upwards towards the night sky, as the spider built it's web on our lamp post.
While there was a moon yesterday, it's impossible to include both the spider and moon on the same shot due to the moon blurring too much and increasing in size, it looks like a big white disc. So I took a moon shot I did last month, and photoshopped it in. I blurred the moon a little to simulate DOF.
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My thinking is the sharper moon will appear more contrasty to the human eye and compliment your "bright" spider.
