I've downloaded your file and I see the following camera settings when I right-click and choose Properties:
Shutter: 1/15 second
Aperture: f/5.6
Focal length: 50 mm
ISO 800
With macro shots, the depth-of-field will become very small (just a few mm) if you choose a larger aperture (= smaller f-stop number). You can see that the DOF is very small in your photo - that's what you get at f/5.6. Try setting the camera on Av mode, which allows you to control the aperture setting (the camera will choose the matching shutter speed to make a correct exposure). Set the aperture to f/16 or f/22 for example, to increase the depth-of-field. Note that the smaller you set the aperture (= the higher the f-stop), the longer the shutter speed will need to be.
f/16 is 3 stops down from f/5.6, so if the light would be the same as your photo above, the shutter speed would need to be 1/2 second (and at f/22, you'd need 1 second).