This is one of those frequently asked questions. Perhaps only the 24-105 vs 24-70 or which version of the 70-200 is asked more. The thing is though, that each person's usage is slightly different, so that an answer for one may not be the answer for all.
That said, there's a versatility with the 100-400 that can't be duplicated with the 300+tc or 400 prime choice. You might check out forum user "lightrules" on pbase to see his tests. I'm pretty sure he shows this test. The 100-400 may duplicate some of your 70-300 focal range, but you could also sell that.
The 400 prime is often said to be the 'bird in flight' lens due to a slightly quicker af. As far as IQ loss or dust, I'm not sure either is true. I'm on my second copy of this wonderful lens and couldn't recommend it highly enough.
Either of the other two solutions, 300+tc or 400 prime, will offer the same aperture at the 400 length as the 100-400. The 300 retains IS and the ability to be a 300. But I think more than one user will tell you that the IQ is less than the zoom. Of course, if you're concern for IQ is because you routinely produce poster-sized prints or you want to see sharpness at 200% on your monitor, you may not be happy with any of your choices here.
100-400 zoom, IS, and terrific IQ and fast AF - what more can you ask for?