I tried it set to off and it just didn't work well with my 100-400. The way I read/understood the manual, in my teeny tiny little noobie brain, I figured "who wants the lens hunting all over the place trying to find focus." Well, my 100-400 would sometimes not even try to focus when going from being focused on a close object to trying to focus on a far object, and the other way around. A call as well as a few emails to Canon and was told this setting was for the long telephoto lenses. They recommended that it be left in the "on" setting for most other lenses. My 40D focuses just fine this way. Happy me.