I switched from the 400/5.6 prime to the 100-400 and have not been sorry for even a minute. It's my belief that the 100-400 is as sharp as my 400 prime was. I shoot it wide open at the full 400mm and am very pleased with the results.
I wanted it primarily for the IS and feel my keeper rate is higher because of it. I have also found that the versatility of the zoom has been a bonus.
Here are some recent samples...
The two Chickadee shots were at 400mm, wide open at 5.6
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This is at least a 50% crop from another hand held shot, stopped down 1/3 stop and a shutter speed of 1/400, ISO1600 (which is not the best for sharpness)
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I thought some actual results would be useful.