The problem with the rebel series is that the camera bodies don't exactly upgrade - they just get less limitations placed upon them as the upper levels of the camera market upgrade. This means that even if it had the same sensor as a 1DMIV the rebel camera body would still have the coded limitations of a rebel - so saying it has the same sensor is not fully the whole picture.
I would expect (though not had a chance to try) that the usable ISO of the 550D would not be far and above that of the 400D and from what the OP says they really want more usable ISOs. I would expect the 550D to give me a possibly cleaner ISO 800 and 400 - but I would be very surprised if you could easily work at ISO 800 with the camera.
If you want that kind of upgrade you have to move up to the next camera body level in the canon series. A 50D or a 40D would give a user about the same level of usable ISO performance (with 50D images being larger) whilst the 7D gives an ISO performance as well as overall AF and body upgrade over the 50D.
However I also stressed the point about lenses - a good camera body is only going to perform as well as the glass it "sees" through so considering the glass that the OP has is an impotant stage. It might be that putting the money toward a more pro end bit of glass would give a far more improved output from the camera body than simply using existing glass on a better body would.




