This is my first telephoto lens over 200mm and I thought I would give my impressions on it having used the lens for a couple of days. I don't really do wall tests or any of that stuff, so I tend to use real world examples and impressions.
Build Quality:
The build is very nice on this lens. It has a very robust feel and appears to be made of mostly metal. I really like the simplicity of the design; there are two focus limiter switches and a mf/af switch and that's about it. The manual focus ring also has an interesting design with the large rubber ring and a smaller ring a little bit farther down the barrel. I actually like this design since my fingers seem to find the smaller ring towards the end in actual use.
I think that the built in hood is a brilliant idea. Since I always shoot with the hood on anyway, it prevents me from having to take it off and reverse it after storage. The mechanism works flawlessly, just push out and then screw to tighten. This goes for the tripod ring as well. You are able to take it off and on without removing the lens which is a very handy feature with a lens that is good for bif. I can be taking picture of an animal, look up and see a bird flying over, unscrew the tripod ring and raise the lens up to shoot handheld. This is design is similar to my Sigma 70-200 and I prefer it to the Canon 70-200 tripod collar.
Autofocus:
Very fast, especially on the 8.5m-infinity setting. Seems to lock on well and is very quiet in operation. I would compare the focus speed to the 70-200 f/2.8 lenses that I have used. I personally find it impressive that this lens can keep up with its f/2.8 cousins being a f/5.6 lens. The AF tracking is very smooth on this lens as well.
In Use
I really like the feel of this lens when using handheld. I find myself limited to strictly daylight usage without a tripod/monopod, but when I can use it handheld it is a joy to use. I am in the military and have a hunting background and using this lens reminds me of shooting. I am able to use my rifle techniques when shooting this lens due to the long, skinny barrel. It feels very light for a lens its size and is easy for me to carry around all day. It will take a while for me to develop skills in framing moving objects quickly at 400mm. I am used to zooming out and then zooming in once I have captured the target, but now I really have to work on my technique and being able to lock on target right away. My technique at this point is: look over the top of my hot shoe, line up on the target, then lower my eye into the viewfinder and raise camera slightly.
Lack of IS: One of the most debatable things about this lens is rather or not it is worth owning due to the lack of IS. I think it is, but does require some technique changes if you are used to using handheld lenses. I previously used my 70-200 f/2.8 for wildlife and found myself shooting handheld a lot of the time due to the f/2.8 aperture. I am now using my tripod pretty much exclusively. I don't really find this a problem since I believe that tripod images have the highest IQ in general anyway and one of the main reasons I chose this lens is for it's IQ reputation. I also am in the woods most of the time and try to keep my ISO 400 or below if possible and 800 at the max, so the tripod is needed due to lack of handholdable shutter speed.
Once I improve my long lens technique, I will probably try more handheld shots. One thing I will say is that while shooting during the day, it should be no problem to get sufficient shutter speeds for BIF shots at ISO 400 or less.
Image Quality:
Sharpness:I have found the image quality of this lens to be excellent. I did a test using a sign and found the lens to be almost exactly the same sharpness from f/5.6-f/8. This is the first lens that I've used where the results were this close (barely beating out my 17-55 which is excellent wide open). This is very nice since it will allow me to keep my ISO values lower and always have as limited a DOF as possible.
Colors etc.:Images are contrasty and have pleasing colors. Comparable to the other Canon L lens that I have used (70-200 f/2.8).
Bokeh: I find the bokeh very nice on this lens. One of the deciding factors on buying this lens was the bokeh quality. I have looked through the entire archive on both this lens and the 100-400 and have always preferred the bokeh of the prime to the zoom. That's not saying that the 100-400 can't provide nice bokeh, I've seen some excellent bokeh from the lens, but that the prime seems to provide more consistent bokeh, especially in busy backgrounds. The zoom bokeh sometimes has a funky look that I don't like, but it's just a personal preference.
Test Images
ISO 400, f/5.6 about a 30% crop
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ISO 400, f/5.6 about a 10% crop
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ISO 800, f/5.6 HEAVY crop
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