I recently updated my iPod Touch to iOS4 and I have also found the UI a bit less responsive. Facebook app doesn't seem to auto update the news feed anymore. I have an aftermarket FM transmitter (Belkin brand) I use in my wifes car and sometimes the iPod pops up a warning saying this device is not supported and doesn't work, haven't had that before. Usually reconnecting it takes care of that.
I haven't noticed a drop in battery life.

