Well, NR10 let's review...
1. For handheld shooting, you should use a shutter speed NO SLOWER than 1/focal length...400mm lens shoulld be shot with a shutter speed of 1/400...some people will tell you that that rule was for 35mm bodies and since the body you have has 1.6x the field of view of a 35mm camera, you should multiply the rule by 1.6...in the case of the 400mm that would be a minimum of 1/640 or so
2. INCREASE your ISO setting to one that allows you to use that shutter speed....but...
3. use an F-stop smaller than wide open...that will give you more depth of field/focus...more in focus in front of and behind the point of focus...You may have to INCREASE ISO to allow you to use F8 or F11 since the smaller F-stop will let in less light (like the faster shutter speed).....
4. You do not need to use the fastest ISO that the camera permits...for most daylight situations, ISO of 1000 will allow you to use 1/500 and F8 (that would be the exposure for a cloudy day or light shade)...if it's darker than that increase the ISO to 1600 before using a slower shutter speed or wider aperture....
5. you will not likely see much noise in a full frame image...yes, if you crop a small section and look at it as an 8 x 10, you will see some, but in photoshop you can still smooth it out a bit...
6. Learn more about RAW processing in that PoTN forum where I posted this recently
about sharpening:
AFter sharpening your RAW image to a level 3-5 in DPP...
For sharpening In Photoshop here's a basic rule regarding USM to follow that might help...
Keeping in mind 2 of the variables, Amount and Radius...(excluding threshold)...and with a starting point of 300% and .3 for each respectively...
Adjusting amount is more subtle than radius...
If the image has few lines and edges, and more texture and tone, like a close-up portrait,
you can use .3 for Radius but you may want to lower to Amount to 200% (adjustments seem take affect I think in 100s: 100, 200, 300...up to 500) or even 100...
You may then increase Radius to .4, .5 or even 1.0 depending on the texture/blocks of tones...this will increase the texture's sharpness nicely...but it ill also increase high ISO noise easily seen in such tonal blocks lacking detail, so be careful there...
But...in the reverse situation, if the image has few solid blocks of tone and texture, but instead has a lot of edges and lines, such as scenics with a lot of trees, (branches and leaves), then you DON'T want high Radius because it will cause ghosting of those edges...in this case you want to LOWER radius to .2 or .1 perhaps, and increase Amount to 300, 400 or 500...this more nuanced sharpening will not cause those edges to break up...
Good luck....
My Canon kit 450D/s90; Canon lenses 18-55 IS, 70-210/3.5-4.5....Nikon kit: D610; 28-105/3.5-4.5, 75-300/4.5-5.6 AF, 50/1.8D Nikkors, Tamron 80-210; MF Nikkors: 50/2K, 50/1.4 AI-S, 50/1.8 SeriesE, 60/2.8 Micro Nikkor (AF locked), 85mm/1.8K-AI, 105/2.5 AIS/P.C, 135/2.8K/Q.C, 180/2.8 ED, 200/4Q/AIS, 300/4.5H-AI, ++ Tamron 70-210/3.8-4, Vivitar/Kiron 28/2, ser.1 70-210/3.5, ser.1 28-90; Vivitar/Komine and Samyang 28/2.8; 35mm Nikon F/FM/FE2, Rebel 2K...HTC RE UWA camera