A medical sample of some kind?

Not quite!

Its actually a paper and plastic CD slip case photographed at 40X. Because of the minimal depth of field when shotting thru a microscope I couldnt get both the surface, with the bubbles on, and the fibrous part below both in focus, so I took two photographs at different focus points and combined them as layers in Photoshop. The two layers overlapped slightly due to focus shift but I left the borders in.

You know about focus stacking software?

While I love to look and photograph the bugs in drops of water, I also have a very active imagination. I see things in abstract, like seeing faces in woodgrain or clouds. Since I got my microscope, I see lot's of things in something as simple as a dried up drop of water.

Could you explain or show how you mount the camera to shoot through your scope? I have ordered a Canon SX130 IS and it's out on the truck for delivery today. Can't wait.

canonloader wrote in post #11101176
You know about focus stacking software?

While I love to look and photograph the bugs in drops of water, I also have a very active imagination. I see things in abstract, like seeing faces in woodgrain or clouds. Since I got my microscope, I see lot's of things in something as simple as a dried up drop of water.

Oh I just hold the camera over the eyepiece and hope!

I saw your post at the other place. You probably know I am on the trail for finding ways to use these cameras through the scope.

I have a 1D MkIII and a couple of adapters for the trinocular port, but so far, I am not much satisfied with the images I get. It's either the quality of my scope, the quality of the objectives, or the adapters themselves. From surfing YouTube and a few forums, I have seen the best images from point and shoot cameras just held up to the ocular lens.

When my latest camera gets here, I'll know more.

canonloader wrote in post #11101270
I saw your post at the other place. You probably know I am on the trail for finding ways to use these cameras through the scope.

Other place?

Photomacrography.net

canonloader wrote in post #11101336
Photomacrography.net

Oh I didnt realise you were a member there too! I've not been on there for a while as I've not been in the mood for macro for ages. So whats your username there?

Mitch640. I'm so new to microscopes that it's pitiful. Cameras I know, but this is all different. My scope is new, and with the eye through the oculars, everything is crystal clear and sharp, but no matter what camera I attach, the images look terrible. Soft, OOF or the color is way off and I can't track it down. I am thinking it might be my Objective lens. They are just Chinese made Achromatics. I am thinking I need some Plan Achromatics and hope they work.

canonloader wrote in post #11101421
Mitch640. I'm so new to microscopes that it's pitiful. Cameras I know, but this is all different. My scope is new, and with the eye through the oculars, everything is crystal clear and sharp, but no matter what camera I attach, the images look terrible. Soft, OOF or the color is way off and I can't track it down. I am thinking it might be my Objective lens. They are just Chinese made Achromatics. I am thinking I need some Plan Achromatics and hope they work.

Well if it looks fine thru the scope but gets bad when you atach the camera then the answer is in the way the camera is attached. I'm no expert on that as I dont have a way of attaching my DSLR to it, I just wing it with an old beaten up chinese monocular (I can only see through one eye so a stereo is pointless)

Well, I will know by later today. This is the 4th or 5th camera I have tried, so I am hoping something works soon. LOL

Do you remove the camera lens when attaching the camera to the scope? (I think thats called afocal but I'm not sure)
Have you queried this on photomacrography.net?

I have tried every possible combination with the adapters and equipment that came with the scope, and using several kinds of cameras. I have a 1D MkIII and two adapters that fit where the lens goes. One with lenses built in and one that you drop one of your eyepieces into then attach to the camera, These both then go onto the trinocular port on the scope. They both do work, but using EOS Utility to connect to the computer and using that to control the setting and trip the shutter, the final images are nothing like what I see with my eyes. And I know for sure, it's not the 1D MkIII, cause it takes perfect shots using regular lenses.

Then, I have bought a 10Mp video and still camera that mounts either on the trinocular port or fits in the eyepiece tube after taking the lens out. This takes fair videos and stills, but again, nothing even close to what I am seeing with my eyes through the eyepieces. I have also tried a 5Mp camera of the same type, and a Sony CCD camera that mounts the same way. The Sony came closest to giving decent images, but it had to run through a TV first, or a converter box, which I have.

The camera on the truck today, the Canon SX130 IS, is also a CCD sensor type, but I have no real good way to mount it to the scope, other than to hold it there or put it on my tripod and try and position it so it shoots down the eye tube.

I have run all this through the other forum to and got some good answers, but still no good images. LOL If I can not get this new camera to mount well, I am going to make an adapter from some 2 part epoxy to attach the camera lens to the eyepiece and see if that works. It's been done before by someone at the other forum. Just a matter of doing it. I do have a Canon handicam type video camera that I got to shoot down the eyepiece, with the lens in the tube. I just positioned it in the right place with my tripod and ballhead, and that worked, but the camera is just a small mega pixel sensor type. The images were OK, but not much detail cause I had to zoom it out pretty far to get it on the sensor.

As you can imagine, this is all pretty frustrating, cause I see others using P&S cameras and getting great video and stills from their stuff.

Its very odd, very odd indeed!

I have narrowed it down to two possibilities, at least 2 that seem plausible.

1. The DSLR sensor just does not like the objective lens coating on my microscope.

2. I can not get high enough shutter speeds to stop the motion and get sharp images with the available light that comes through the scope.

Or, it could be both.

My new camera is out for deliver right now, so I will know if it's the shutter speed, or the vibration from the shutter today. The new camera does not use a shutter, so no vibration. I will let you know the outcome, cause I know you were having problems too. Maybe between us, we can track it down.

What light are you using? I mean I can get away with any light source i have to hand including wind up LED torches, phone lights etc.